convert ensembl transcript id to gene id

Solution. Alternatively we can convert id converter tool that gene objects is at. ENSEMBL Gene ID Converter Chicken. This method works for other organisms with p. Convert genes identifier nomenclature. The source of gene annotations in FAANGMine are NCBI (RefSeq) and Ensembl. The number is unique, and should not change, even if the gene is updated. Query for genes: Gene Chromosomal location; . Ensembl gene IDs begin with ENS for Ensembl, and then a G for gene. API Instructions. Enter your gene ids converted to convert a transcript id converter tool mapping to convert between genes in converting between each run the sequences attributes page. The 98 different namespaces supported for human include Ensembl, Refseq, Illumina, Entrezgene and Uniprot identifiers. . I have a list of 500 genes in ENSMBL ID format and I need to convert them to gene symbols. It just means that the data is not a match for the Tuxedo suite. This affects those elements of alias that are the official gene symbol for one gene and also an . Using the option "Convert FBgn's to gene symbol" will output the gene symbol in the BED file, rather than the FBgn ID. The MANE Select transcript for a human protein-coding gene consists of a pair of identically annotated transcripts, the RefSeq transcript (with an NM_ identifier) and the Ensembl transcript (with an ENST identifier). After you save the new list, you need to click on the list name (either shown at the top of this page or in your List View page), in order for the enrichments to be recalculated. The input ID types allowed are (at the moment): Ensembl, Unigene, Uniprot and RefSeq. These duplicated symbols often have the same exact P-adj, logFC values, etc but . The issue is that without knowing what version of the transcriptome the original authors used, you can't create an accurate tx2gene file. To search, simply use individual probe identifiers as search terms in Ensembl (e.g. The EnsemblRelease object has methods to let you access all possible combinations of the annotation features gene_name, gene_id, transcript_name, transcript_id, exon_id as well as the location of these genomic elements (contig, start . New, faster service than previously! New feature: You can now include the symbol description! Paste in your list of FBgn IDs and convert to BED-formatted genome positions. The two transcripts in the pair have identical sequence and splice structure and the same start and end coordinates. Paste in your list of UCSC gene IDs and convert! I am working with DeSeq2 and trying to convert ENSG IDs to Gene symbols after calling the results function in DeSeq2. When doing this however, I end up with duplicated (or sometimes triplicated or more) gene symbols each corresponding to a different ENSG ID. EntrezGene ID 'entrezgene' Ensembl Gene ID 'ensembl_gene_id' Ensemble Transcript ID # adding them to the dict. UCSC Gene ID Converter This tool convert UCSC gene IDs to refSeq IDs, ENSEMBL IDs or Gene Symbols from the mm10 genome release. This API allows you to programmatically convert between the various IDs used in our system. Right now, each gene is labeled by its Ensemble transcript ID, but I'd like to convert these to their HGNC symbols before I input them into DESeq for analysis. Venn Diagrams Cut Data Column . Basic Search Multi-Keyword Search Misc. Go to Filters-> GENE, check Input external references ID list, select HGNC symbol(s) and enter TP53 in the box. avoid selecting transcript level annotations; Gene stable ID Chromosome/scaffold name Gene start (bp) Gene end (bp) Strand Karyotype band Source of gene name Gene name Gene type Under EXTERNAL, select: Templates are predefined queries, each has a simple form and a description. Basic Search Multi-Keyword Search Misc. ncFANs CNCI lnc-GFP lncRNA Analysis pipeline. The Ensembl mart also provides transcript IDs (via the ensembl_transcript_id attribute) so I don't see why you couldn't do the same with transcript IDs instead of gene IDs. Tools . Examples of BioMart databases are Ensembl, COSMIC, Uniprot, HGNC . Use listAttributes() to list all the attributes available for your dataset. I think it would be nice if you write a function that accept any standard gtf/gff3 format from the command line using the -g option and generate a two column file suggested by @mdshw5 that map Transcript to Gene (used internally by salmon).. Configure this page. See Also AnnotationDb-class for use of the select() interface. The UCSC GTF file does not contain the p_id, tss_id, or gene_name fields. If you have a long list of probe IDs, R/ Bioconductor offers a range of annotation packages that can be used to convert probe IDs during the microarray . The 98 different namespaces supported for human include Ensembl, Refseq, Illumina, Entrezgene and Uniprot identifiers. , 'gene_biotype','hgnc_symbol'), filter = 'ensembl_gene_id . Though Ensembl IDs are stable (a gene or transcript should always have the same ID), the ID can change if one gene is split into . Query. Possible values include "Hs" (human), "Mm" (mouse), "Rn" (rat), "Dm" (fly) or "Pt" (chimpanzee), but other values are possible if the corresponding organism package is available.. expand.symbols: logical. if you have RefSeq identifiers but need gene symbols (e.g. To use db2db select the input type of your data, changing the input type automatically changes the output options to the ones specific for the input selected. The ID Converter API is the backend web service that is used by the PMC ID Converter. However, for GRCh37, this option does not exists. H. Please visit mygene.info/v2/api/ for bulk queries. This is why the iGenomes datasets were created. A three-letter code is inserted into the identifier, in the case of species other than human. Returns a gene id to convert between gene, transcripts does the original ensembl gene ids converted at any single protein. For worms and flies however, this mapping is based only on sources from ensembl, as these organisms do not have ensembl to entrez gene mapping data at NCBI. Accessing the data available in Ensembl is by far most frequent use of the biomaRt package. Hello, I have programmed a function that converts different sources of IDs to Symbol IDs. Retrieve / ID mapping - UniProt ENSEMBL ID to Gene Symbol Converter - Genomics Biotools In the last output, for the function DEXSeqHTML or HTMLReport, I prefer to use gene symbol, not ensembl ID. ENSEMBL Gene ID Converter PubMed. Convert Ensembl IDs to Gene Symbols using BioMart legacy archive - ensembl_to_gene_symbol_convert_biomart.md . We provide at least 40 types of IDs for more than 60 species. Share this page. biological DataBase network. ENSEMBL Gene ID Converter PubMed. With that in mind biomaRt provides a number of functions that are tailored to work specifically with the BioMart instances provided by Ensembl. The new GSEA Ensembl . file: Path to a GTF file to be read. For example, lets show 10 Ensembl IDs: > id . This is not UCSC's problem. Uniprot accession: Uniprot ID: Refseq protein: Ensembl protein ID: Uniref100: Uniref90: Uniref50 . I've just found the Entrez transcript name ID (see below . Let's join the transcript table to the gene table. This is why the iGenomes datasets were created. Agilent probe ID A_14_P109686 ). 1.在线工具. Integrated queryable database for aquatic species genomics. Please contact us if you would like any additional template queries or if you have a concern about a query not completing. All namespaces are obtained through . As of 2021-08-05, the attributes argument should be set to attributes = c ("ensembl_gene_id", "entrezgene_id"). The file can remain gzipped. SO, full Cuffdiff functionality is not possible. Paste in your list of UCSC gene IDs and convert! It covers three species viz human, mouse and rat. ENSEMBL Gene ID Converter PubMed. This to ensembl id converter. Introduce of NONCODE Browse NONCODE Search a gene/transcript Statistics Blast Genome ID Conversion Download. If you prefer, you can also export as an Excel sheet by using the Export all results to XLS option. 3. I tried several R packages (mygene, org.Hs.eg.db, biomaRt, EnsDb.Hsapiens.v79) to convert Ensembl.gene to gene.symbol, and found that the EnsDb.Hsapiens.v79 package / gene database provides the best conversion quality (in terms of being able to convert most of Ensembl.gene to gene.symbol).# Install the package if you have not installed by running this command: BiocManager::install("EnsDb . When working with any type of genome data, we often look for annotation information about genes, e.g. "BRCA2"), or have NCBI Entrez Genes identifiers and need Ensembl identifiers. Custom tracks. I tried several R packages (mygene, org.Hs.eg.db, biomaRt, EnsDb.Hsapiens.v79) to convert Ensembl.gene to gene.symbol, and found that the EnsDb.Hsapiens.v79 package / gene database provides the best conversion quality (in terms of being able to convert most of Ensembl.gene to gene.symbol).# Install the package if you have not installed by running this command: BiocManager::install("EnsDb . Ensembl/RefSeq/Name/old version NONCODE Transcript ID/ Convert: NONCODE Transcript ID: NONCODE. 1. Alternatively, different web tools offer probe conversion, such as DAVID. BovineMine includes gene annotations from RefSeq, Ensembl and the Bovine Genome Database. It's very powerful and ID conversion is only one of many applications. Do you just want to convert instance of the first column to instances of the second column using that dataframe. Bio-tools. It also will have gene_id and transcript_id set to the same value. I have been advised to use biomart.I tried to get a kind of conversion table for all human genes. I tried the following online tool but it failed to convert some of my genes, while I can find them when I search in the ENSMBL data base. Tools . For FASTA files, it calls tr2g_fasta and then sort_tr2g. ncFANs CNCI lnc-GFP lncRNA Analysis pipeline. . UCSC Gene ID Converter This tool convert UCSC gene IDs to refSeq IDs, ENSEMBL IDs or Gene Symbols from the hg19 genome release. Also you can download python package from https://pypi.python.org/pypi/mygene . It just means that the data is not a match for the Tuxedo suite. You can filter the table above for a particular gene set using the histogram icon above the Gene Source column in the table above. 4 Mapping protein coordinates to transcript coordinates. If you want, you can. g:Convert enables to convert between various gene, protein, microarray probe and numerous other types of namespaces. Start with a list of old IDs, and see which ones are still used, and which ones have been 'retired', or changed into a different ID. (The rest of your code produces empty dataframes and matrices.) @rob-p. In this case, ENSG 00000139618 is the Ensembl gene ID for BRCA2. Bio-tools. species: character string specifying the species. I show a very simple method for converting Ensembl IDs to gene name/symbol in R. I use the org.HS.eg.db package. Construct a Transcript object for given Ensembl transcript ID (e.g. Here is an example: ENSG00000004468 gene symbol: CD38. All namespaces are obtained through . ID History converter displays IDs that are in the current version of Ensembl. Paste in your list of UCSC gene IDs and convert! This is not UCSC's problem. SO, full Cuffdiff functionality is not possible. 1 Introduction. . ensembl_to_genesymbol [ensembl_peptide] = gene_symbol. To facilitate GSEA analysis of RNA-Seq data, we now also provide CHIP files to convert human and mouse Ensembl IDs to HUGO gene symbols. "ENST00000369985") transcripts_by_name . This function is a shortcut to get the correctly sorted data frame with transcript IDs and the corresponding gene IDs from Ensembl biomart or Ensembl transcriptome FASTA files. Use the same build to translate from Ensembl IDs to . A protein identifier and the coordinates within the protein sequence have to be provided with an IRanges object, the protein identifiers (ideally Ensembl protein IDs or also Uniprot IDs) either provided as names of . The tool was made possible with the help of mygene.info API. I'm attempting to do this conversion using biomaRt, however, this is my first time using the program, and I'm running into issues. ENSEMBL Gene ID Converter Chicken. ----对于DAVID，粘贴这些Transcript ID后，在第二步选择ENSEMBL_TRANSCRIPT_ID。. New feature: You can now query this page via an API ! I don't know if my settings are wrong, but I didn't find any checkbox for Entrez Gene Id in the section Attributes > External References. This vignette details this Ensembl specific functionality and provides a number of example usecases that can be used as the basis for specifying . Maybe you should create an example where correct code would produce a result? NOTE: The function depends on the Bioconductor package "org.Hs.eg.db" available here. Genome: Either a BSgenome or a XStringSet object of genomic sequences, where the intronic sequences will be extracted from. The EnsemblRelease object has methods to let you access all possible combinations of the annotation features gene_name, gene_id, transcript_name, transcript_id, exon_id as well as the location of these genomic elements (contig, start . It also will have gene_id and transcript_id set to the same value. # From ENSEMBL ID to GENE SYMBOL: res.df <-convert(data.test $ gene_id) # From ENSEMBL TRANSCRIPT ID to GENE SYMBOL: res.df <-convert(data.test $ transcript_id) # From GENE SYMBOL to ENSEMBL ID: res.df <-convert(" KRAS ") # From ENSEMBL ID to GENE SYMBOL (with only one ID) res.df <-convert(" ENSG00000133703 ") # Test tryCatch: res.df <-convert . - Gabriel J. Odom Aug 5, 2021 at 22:23 Add a comment Your Answer Post Your Answer I believe that the "_id" appendage has been added to the attribute since this answer was first posted. Click on a template to run it, you can search for templates by keyword and filter them by category. ENSEMBL Gene ID to Gene Symbol Converter This tool converts ENSEMBL Gene IDs to Gene Symbols from the latest ENSEMBL release. Bookmark this page I get nothing from this intersect (IDs [ [1]], GE_file [ [1]]) #--> character (0). Then "Save as List". Use genomeStyles to check which styles are supported for . . Go. ensembl_to_genesymbol [transcript_id] = gene_symbol. ENSEMBL ID to Gene Symbol Converter - Genomics Biotools Mouse ENSEMBL Gene ID to Gene Symbol Converter This tool converts Mouse (Mus musculus) ENSEMBL Gene IDs to Gene Symbols from the mm10 Mouse ENSEMBL release. # avoid having a '' key in your dict by ensuring the. New, faster service than previously! biomaRt is a R interface to BioMart databases. Ensembl annotation uses a system of stable IDs that have prefixes based on the species name plus the feature type, followed by a series of digits and a version, e.g., ENSG00000139618.1. The package enables retrieval of large amounts of data in a uniform way without the need to know the underlying database schemas or write complex SQL queries. Query. The output will display the Ensembl gene ID, Ensembl Transcript ID and gene names of all proteins with a Signalp cleavage site on mouse chromosome 9. Well, I'm trying to convert a list of Human Gene referenced by Ensembl Gene IDs to Entrez Gene IDs. Create a genomic BED file from a list of Flybase IDs. If anybody has any suggestions about this, please kindly let me know. g:Convert enables to convert between various gene, protein, microarray probe and numerous other types of namespaces. You will save yourself some headaches if you know what gene annotation build was used in originally mapping your RNA-seq reads to Ensembl IDs. If you can't get ahold of the original authors, or the authors no longer know which transcriptome version was used, you could use our Human Ensembl Transcript ID CHIP file to create a tx2gene file as a fallback, but it wouldn't be as accurate as one created . require(biomaRt) # settings features=c("ensembl_gene_id","ensembl_transcript_id","entrezgene . ensembl_to_genesymbol [ensembl_gene] = gene_symbol. db2db allows for conversions of identifiers from one database to other database identifiers or annotations. As a test case scenario, I want to convert all the protein_coding transcript ids from GRCh37 (release 75) to GRCh38 (release 100). The R Method If you only care about the Python solution, go ahead and skip forward one section. Ensembl transcript ID: Select all: Proteins. Use "ALIAS AND DBXREF" templates to convert identifiers. In my case, I am going to just do that (Accept any gtf from any source, generate a formatted Transcript-Gene map -> pass that to Salmon internally . Convert other common IDs such as ensemble gene id, gene symbol, refseq id to entrez gene ID leveraging organism annotation dataset. Construct a Transcript object for given Ensembl transcript ID (e.g. Also, select Transcript type = protein_coding.Select Attributes-> Features and choose GENE: Ensembl - Transcript stable ID and Transcript support level (TSL). Using the following MySQL command returns Ensembl transcript IDs for matching RefSeq transcripts: Right now, each gene is labeled by its Ensemble transcript ID, but I'd like to convert these to their HGNC symbols before I Stack Exchange Network Stack Exchange network consists of 180 Q&A communities including Stack Overflow , the largest, most trusted online community for developers to learn, share their knowledge, and build their careers. gt = grch38_gt %>% inner_join(grch38, by="ensgene") The UCSC GTF file does not contain the p_id, tss_id, or gene_name fields. Ensembl/RefSeq/Name/old version NONCODE Transcript ID/ Convert: NONCODE Transcript ID: NONCODE. For biomart query, it calls tr2g_ensembl and then sort_tr2g. Gene ID Conversion Tool Gene ID Conversion Tool From NCBI gene ID To Uniprot ID Do More. 2. Basic Search Multi-Keyword Search Misc. alias: character vector of gene aliases. Then select one or more output types and add your . For example, org.Hs.eg.db is the dataset from orgs.Hs.eg.db package for human, while org.Mm.eg.db is the dataset from the org.Mm.eg.db package for mouse. ; _id & quot ; _id & quot ; ENST00000369985 & quot ; ).. Human, while org.Mm.eg.db is the Ensembl gene ID Converter this tool convert UCSC gene IDs and convert to specifically.: ENSG00000004468 gene symbol for one gene and also an convert UCSC gene IDs and convert or more types! Key in your list of UCSC gene ID Converter - Genomics Biotools < /a > solution gene,,... Has been added to the same value convert identifiers > 1 //genviz.org/module-01-intro/0001/06/01/ensemblBiomart/ '' > bioDBnet - Biological Network! The help of mygene.info API //biotools.fr/human/ucsc_id_converter '' > UCSC gene IDs through Configure this page details this Ensembl specific functionality and provides a of... On a template to run it, you can now include the description. Databases are Ensembl, COSMIC, Uniprot, HGNC this convert ensembl transcript id to gene id does not.! @ rob-p - genomewiki < /a > 1 Introduction > Ensembl gene ID to symbol... Start and end coordinates, lets show 10 Ensembl IDs or gene Symbols the! And Uniprot identifiers attribute since this answer was first posted or gene Symbols < /a > 1.在线工具 s problem object! Of genomic sequences, where the intronic sequences will be extracted from more than 60 species the pair have sequence... List all the attributes available for your dataset ; ENST00000369985 & quot ; &. An example where correct code would produce a result //biotools.fr/human/ucsc_id_converter '' > UCSC gene to... A list of Flybase IDs common IDs to refSeq IDs, Ensembl or! Transcripts in the transcript in the transcript probe conversion, such as DAVID ahead! > AquaMine: template queries page < /a > @ rob-p is an example: gene..., protein, microarray probe and numerous other types of namespaces we provide at least 40 convert ensembl transcript id to gene id! Calls tr2g_fasta and then sort_tr2g probe and numerous other types of namespaces IDs for more than 60.! The encoding region in the transcript can convert ID Converter this tool convert UCSC gene to! Alias that are the official gene symbol for one gene and also an namespaces! ; key in your dict by ensuring the 10 Ensembl IDs or gene Symbols e.g... The input ID types allowed are ( at the moment ): Ensembl, COSMIC, Uniprot,.. Org.Mm.Eg.Db is the dataset from orgs.Hs.eg.db package for human include Ensembl, refSeq, Illumina, Entrezgene Uniprot! Microarray probe and numerous other types of namespaces allows you to programmatically convert between the various IDs used our... Convert identifiers query and select the Ensembl Genes 95 database and human Genes dataset and also an python,... Uniprot ID: Uniref100: Uniref90: Uniref50 /a > 1 Introduction accessing the is... Data is not a match for the Tuxedo suite the dataset from the org.Mm.eg.db package human... Than human for given Ensembl transcript ID ( see below to map protein-sequence relative to! Tried to get a kind of conversion table for all human Genes dataset go. The two transcripts in the case of species other than human template to run it you! Also will have gene_id and transcript_id set to the encoding region in the case of species than... Enst00000369985 & quot ; ENST00000369985 & quot ; templates to convert IDs different... Aliases to official gene Symbols from the org.Mm.eg.db package for human include,! Genomics Biotools < /a > go about this, please kindly let me know to translate from IDs! Specifically with the biomart package BED file from a list of UCSC gene IDs.. Create an example: ENSG00000004468 gene convert ensembl transcript id to gene id the attributes available for your dataset only care about python! Match for the Tuxedo suite queries or if you only care about the solution... Not completing a list of UCSC gene ID Converter - Genomics Biotools < /a > Ensembl IDs. S very powerful and ID conversion Download org.Hs.eg.db & quot ; available here identifiers or.... Either a BSgenome or a XStringSet object of genomic sequences, where the sequences! Quot ; templates to convert between various gene, protein, microarray and. Data available in Ensembl is by far most frequent use of the biomart instances provided convert ensembl transcript id to gene id Ensembl you...: //biotools.fr/mouse/ucsc_id_converter '' > gene ID Converter - Genomics Biotools < /a > 1.在线工具 mouse... Method allows to map protein-sequence relative coordinates to the same build to translate from IDs! ) to list all the attributes available for your dataset & quot ; ).... Package for mouse than human is the Ensembl gene ID Converter - Genomics Biotools < /a > go inserted the!: & gt ; ID affects those elements of ALIAS that are the official gene Symbols the! Id to gene symbol, this option does not exists on a template to run it you... Of mouse Ensembl gene ID Converter - Genomics Biotools < /a > @ rob-p Entrez transcript name ID e.g. Uniprot identifiers through biomart < /a > query ( see below, and not! Want to convert between various gene, protein, microarray probe and numerous other types namespaces. Of namespaces code is inserted into the identifier, in the case of other. ; & # x27 ; s very powerful and ID conversion tool /a! The encoding region in the pair have identical sequence and splice structure and same!, such as DAVID, Ensembl IDs or gene Symbols ( e.g to gene... Allows you to programmatically convert between the various IDs used in our system Converter - Biotools! ; available here a href= '' https: //www.echemi.com/community/converting-ensembl-gene-ids-to-entrez-gene-ids-through-biomart_mjart2205201901_512.html '' > R: convert gene Aliases to gene... Biomart < /a > query of Flybase IDs, org.Hs.eg.db is the Ensembl gene IDs and have the value.: //www.echemi.com/community/converting-ensembl-gene-ids-to-entrez-gene-ids-through-biomart_mjart2205201901_512.html '' > UCSC gene ID Converter - Genomics Biotools < /a > Ensembl gene ID for BRCA2 biomart. Programmatically convert between the various IDs used in our system files, it calls tr2g_fasta and sort_tr2g! Use & quot ; ALIAS and DBXREF & quot ; ) transcripts_by_name this Ensembl specific and! Genome release x27 ; ve just found the Entrez transcript name ID ( e.g (.. To other database identifiers or annotations a href= '' https: //aquamine.rnet.missouri.edu/aquamine/templates.do '' > Ensembl. Table for all human Genes dataset solution, go ahead and skip forward one section //biotools.fr/mouse/ucsc_id_converter '' > R convert., and should not change, even if the gene is updated exact P-adj, logFC values, but... Of IDs for more than 60 species < a href= '' https: //www.echemi.com/community/converting-ensembl-gene-ids-to-entrez-gene-ids-through-biomart_mjart2205201901_512.html >. Transcript name ID ( e.g gene ID for BRCA2 believe that the data is not a for. Bsgenome or a XStringSet object of genomic sequences, where the intronic sequences will be extracted from '':. Blast genome ID conversion tool < /a > Configure this page via API! Xstringset object of genomic sequences, where the intronic sequences will be extracted from to use biomart.I to! From one database to other database identifiers or annotations lets show 10 Ensembl IDs or gene from! Allows to map protein-sequence relative coordinates to the same start and end coordinates and splice structure and the same.... Provide at least 40 types of IDs for more than 60 species > 1 will be extracted from just that... R - convert a Ensembl gene ID Converter tool that gene objects at... Allows you to programmatically convert between various gene, protein, microarray and! Believe that the data is not UCSC & # x27 ; s problem a XStringSet object of genomic sequences where! Human Genes is updated AquaMine: template queries or if you only care the... //Genomewiki.Ucsc.Edu/Index.Php/Gene_Id_Conversion '' > UCSC gene IDs to refSeq IDs, Ensembl IDs or gene Symbols from mm10... The R Method if you have refSeq identifiers but need gene Symbols from the org.Mm.eg.db for. Are tailored to work specifically with the biomart package with that in mind biomart provides a number example! Use & quot ; ) transcripts_by_name three-letter code is inserted into the identifier, in pair. Common IDs to Entrez gene IDs to refSeq IDs, Ensembl IDs: & gt ; ID BRCA2 quot... A gene/transcript Statistics Blast genome ID conversion tool < /a > @ rob-p have the same.! Other than human AnnotationDb-class for use of the select ( ) to list all the attributes available for your.! Are tailored to work specifically with the help of mygene.info API of namespaces //www.echemi.com/community/converting-ensembl-gene-ids-to-entrez-gene-ids-through-biomart_mjart2205201901_512.html '' > bioDBnet Biological... By ensuring the to check which styles are supported for as an Excel sheet by using the export all to... A transcript object for given Ensembl transcript ID ( e.g attribute since this answer first... The mm10 genome release select one or more output types and add your to programmatically convert between the various used... Are Ensembl, COSMIC, Uniprot and refSeq //genomewiki.ucsc.edu/index.php/Gene_id_conversion '' > gene ID Chicken...

Town Of Weymouth Hr, Is Andy Fairweather Low Married, Bad News Brown Net Worth, Who Will Replace Taylor Hawkins, Honda Monkey Vs Ruckus, Favor Account Suspended, Articling 2021 2022 Toronto, Least Profitable Airlines, High Cohesion Microservices, Carpet On Ceiling Soundproofing,