The Cytek Aurora’s use of full spectrum flow cytometry combined with the SpectroFlo ® software’s real-time unmixing capability provides greater fluorochrome choice and panel flexibility and allows users to quickly visualize data and statistics. This guide provides information on the Aurora flow cytometer, daily workflow, SpectroFlo® software features, instrument maintenance, and cytometer specifications. In designing a multicolor panel, you should look for dyes with unique spectra and consider their impacts on other dyes (see slide 18). Cytek® Aurora Fluorochrome Selection Guidelines 2 Laser 16V-14B N9-20021 Rev. Cytek Aurora Fluorochrome Selection Guidelines ... Cytek Aurora Fluorochrome Selection Guidelines Fluorochrome. BioLegend Aurora Spectra Analyzer. Newly build spectral flow cytometer with a unique capability of detection over the full emission spectra. Watch your multicolor panels grow with Spark Dyes, a family of small, synthetic fluorophores. Our affordable spectral flow cytometry instruments make complex applications possible. Contact Caisheng Lu. N9-20031 Rev. 405, 488 and 640nm lasers; 38 channels, 30+ unique fluorochrome signatures, 8 fluorescent protein signatures; Operation manual and fluorochrome selection guideline available. Brightness Index chart) Typically, PE is always the brightest fluorochrome, except in some cases where cells have particularly high autofluorescence, APC may yield the brightest fluores-cence. 2. Operation manual and fluorochrome selection guideline available. Cytek aurora user manual pdf free printable online However, the goal of robust panel design through selection of antigen-fluorochrome combinations while minimizing fluorescent spillover and the resulting spreading to the negative adjacent channels became even more challenging. N9-20017 Rev B Stain Index Ranking: 114 Dyes 14 Bars are color -coded by primary excitation laser(s): UV, Violet, Blue, Yellow-Green, Red. Cytek AuroraTM Fluorochrome Selection Guidelines for 4 Laser Systems Last Updated: October 24, 2018 Fluorochrome Signatures Dyes can be used in combination as long as they have a unique spectrum signature. Full Cytek Fluorochrome Selection Guideline Cytek’s guide to fluorophores on the Aurora – see expected signatures, brightness indexes, spread matrix, etc. Fluorescent Dyes Brochure booklet size cp20141020 .Technical Notes on Fluorescence A = photon absorp. 2 Cytek Biosciences, Inc. 46107 Landing Parkway, Fremont, CA 94538 1-877-922-9835 [email protected] Cytek® Aurora Say Hello to a New Reality The Cytek® Aurora offers unprecedented assay flexibility and panel complexity thanks to our unique, full spectrum optical design. Leave the tube of DI water on the SIP. Aurora users can find many links within the Resources page from panel design tools to helpful YouTube videos demonstrating various instrument and software capabilities. A2 Cytek Aurora Primary Channels for Common Fluorochrome. N9-20017 Rev B Stain Index by Primary Excitation Laser 15 The Emory Pediatrics/Winship Flow Cytomtery core recently purchased 2 addtional Cytek Aurora's (5-Lasers, matched), these were installed in Sep 2021 and Dec 2021 , one is located in the main core lab (HSRB E-362) and the second one in our lab located in Winship Buidling B on the 3rd floor (Room - B3202). Cytek Aurora Spectra Analyzer. Uploaded 07-01-2021 30 Color Spread Matrix with Percentages How to read this table: the fluor in the row impacts the one in the column. Includes fluorochromes that can be used in each fluorescence channel as well as spectral overlap and spectral spreading information to aid in high parameter cytometry panel design. The Cytek Aurora cytometer is an air-cooled, small benchtop instrument. Laser. Member Pubs. Cytek Aurora Detectors Properties. The state-of-the-art optics and low-noise electronics provide excellent sensitivity and resolution. Flat-top beam profiles, combined with a uniquely designed fluidics system, translate to outstanding performance at high sample flow rates. Description. Online spectrum viewer 3. Download the fluorochrome guide that matches your Cytek cytometer configuration. Spectral Flow Cytometry provides an alternative way to measure fluorescence emission. Choose the brightest fluorochrome for your least expressed protein and the dimmest fluoro-chrome for your most highly expressed protein. No changing optical filters for any fluorochrome. Guide to choosing fluorochromes for use on the Cytek Aurora 5 Laser Spectral Cytometer. The 4 laser (16UV-16V-14B-8R) Cytek Aurora can analyze up to 54 fluorochromes, 57 parameters (FSC, 488 SSC, 405 SSC-B). Emission Wavelength (nm) UV Violet Blue Yellow Green Red 395 BUV395 420 BV421 440 Super Bright 436 450 Live/Dead Blue eFluor 450 480 … Cytek® Aurora Fluorochrome Selection Guidelines 4 Laser 16UV-16V-14B-8R N9-20031 Rev. The Cytek Aurora recently received a UV 355nm (20mW) laser upgrade. A Fluorochrome Signatures Dyes can be used in combination if they have unique spectrum signatures. Cytek Aurora Users Community. It also includes troubleshooting tips and service information. A Fluorochrome Signatures Dyes can be used in combination if they have unique spectrum signatures. Cytek® Aurora CS Brochure - Protected (PDF, 6 MB) The Cytek Aurora CS was designed to leverage the paradigm-shifting technology onboard the Cytek Aurora system and deliver the sorting capabilities you have come to expect in a high-end sorter. ... Fluorochrome selection guidelines 2. Receive … Contact Caisheng Lu; Location: PS17-503 ... enabling the use of a wide array of new fluorochrome combinations without reconfiguring the system for each application. The Cytek Aurora CS was designed to leverage the paradigm-shifting technology onboard the Cytek Aurora system and deliver the sorting capabilities you have come to expect in a high-end sorter. Members. Cytek® Aurora cFluor™ Selection Guidelines 5 Laser 16UV-16V-14B-10YG-8R. Cytek Aurora. Safety Safety Symbols The Aurora is intended for research use only; not for diagnostic or therapeutic procedures. Aurora System The Aurora system consists of the Aurora flow cytometer and a computer workstation running SpectroFlo software for acquisition and analysis. A New Level of Accessibility A powerful, high value system that is accessible to a wide range of users. Channels MFI Violet (16 channels) Blue (14 channels) Red (8 channels) Yellow Green (10 channels) This dye is excited by all 5 lasers. A2 Cytek Aurora (with 5L similarity matrix) Configuration ... Additional optimal fluorochrome options available on FluoroFinder.com. Contrary to conventional flow cytometry which measures a limited bandwidth of the emission spectrum of each fluorochrome, spectral flow cytometry measures the entire spectral signature emitted from each of the 5 lasers. 3. Cytek’s fluorochrome guides provide examples of many dyes full spectrum signatures along with stain indices, spread information, and other useful information. BioLegend Aurora Spectra Analyzer. 10x Genomics Chromium. Directions. Individual components from this pdf are saved below. Follow the prompts to load a tube with 3 mL of 10% bleach, followed by a tube with 3 mL of DI water, followed by 3 mL of 30% contrad, followed by 3 mL of DI water. Download Aurora 5 Laser Fluorochrome Guide. " The Cytek Aurora flow cytometer has been an amazing tool for my osteoimmunology research. This instrument was my introduction to flow cytometry completely, and I have had a pleasant experience learning to use it. With the help of my mentor and our core staff, I have run into few issues. The Cytek Aurora is a 5 lasers spectral cytometer. The addition of these expertly-crafted dyes to BioLegend’s portfolio provides researchers with the ability to fill spectral spaces between existing fluorophores, maximizing panel flexibility. Due to the ever evolving technology and applications associated with Flow Cytometry, please discuss your … Beckman Coulter Optima XPN … N9-20021 Rev. Example: 35 Fluorochrome Selection Approx. 2. Cytek® Aurora cFluor Selection Guidelines 5 Laser 16UV-16V-14B-10YG-8R N9-20017 Rev B Cytek® Aurora 5 Laser 16UV-16V-14B-10YG-8R N9-20017 Rev B How to Read Full Spectrum Fluorochrome Signatures 2 Violet (16 channels) Blue (14 channels) Red (8 channels) Yellow-Green (10 channels) This dye is excited by all 5 lasers. This makes it possible to now run 40 colors in one tube, a first for 40 fluorescence-based flow cytometry. In the Cytometer menu from either the QC & Setup or Acquisition module, select Fluidics Shutdown. Cytek Aurora Resources Page. Cytek Aurora Spectra Analyzer. 3. 2 Blue Laser Excitable Dyes 3 The system is intended to be used for analyzing cells in the fields of immunology, biochemistry, biology, oncology, and hematology research. Cytek Aurora Users Community. With up to 5 lasers and 64 fluorescent detectors, the Cytek® Aurora can capture the entire emission spectrum from each fluorochrome. Cytek Aurora Fluorochrome Selection Guide. 4 anding arkway, Fremont, CA 48 -8--8 salescytekbio.com Cyte Aurora Say ello to a ew eality When designing a multicolor panel, you should look for dyes with unique spectra and also consider spread introduced by the dyes (see slide 25). Maximum Accessibility A powerful, high value system that is accessible to a wide range of users. Use any commercially available fluorochrome excited by the onboard lasers. Cytek Aurora Resources Page. Meet Aurora: 2 Cytek iosciences, nc. Use any commercially available dye excited by the lasers onboard. 1. Based on this information, expect this dye to introduce spread into dyes emitting at similar wavelengths. The Cytek Aurora leverages full spectrum technology to provide unprecedented flexibility to scientists, enabling the use of a wide array of new fluorochrome … Cytek Aurora. In this section we provide protocols, data sheets to organize your samples, and fluorochome selection guides to assist in your experimental design. SpectroFlo® QC beads are also included. Cytek Aurora Fluorochrome Selection Guidelines Fluorochrome Signatures Dyes can be used in combination as long as they have a distinct spectrum signature. Aurora users can find many links within the Resources page from panel design tools to helpful YouTube videos demonstrating various instrument and software capabilities. With up to three lasers (red, blue and violet), three scattering channels, and more than 30 fluorescence channels, the Aurora suits every laboratory’s needs, from simple to high complexity applications. Description. Look for dyes with unique spectra and consider spread introduced by the dyes when designing multicolor panels (see slide 27).
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